This research is aimed at assessing the bioactivity of different solvent extracts of the plant followed by annotation of its phytoconstituents. Even though bioassay-based studies indicated the true therapeutic potential of this plant, compound annotation was not performed extensively. Previous studies conducted in this plant are more confined to extract level. Figure S38: Fragmentation pattern of trihydroxy dimethoxyflavone.Īcacia catechu (L.f.) Willd is a profoundly used traditional medicinal plant in Asia. Figure S37: Fragmentation pattern of isorhamnetin. Figure S36: fragmentation pattern of prodelphinidin B. Figure S35: Fragmentation pattern of prodelphinidin B3. Figure S34: Fragmentation pattern of avicularin. Figure S33: Fragmentation pattern of kaempferol. Figure S32: Fragmentation pattern of chrysin. Figure S31: Fragmentation pattern of diosmetin. Figure S30: Fragmentation pattern of isoquercetin. Figure S29: Fragmentation pattern of naringenin. Figure S28: Fragmentation pattern of emodin. Figure S27: Fragmentation pattern of gallocatechin/epigallocatechin. Figure S26: Fragmentation pattern of catechin/epicatechin. Figure S25: Mass spectrum of trihydroxy dimethoxyflavone. Figure S24: Mass spectrum of isorhamnetin. Figure S23: Mass spectrum of pterocarpan. Figure S21: Mass spectrum of aciculatinone. Figure S17: Mass spectrum of prodelphinidin B. Figure S16: Mass spectrum of prodelphinidin B3. Figure S10: Mass spectrum of isoquercetin. Figure S8: Mass spectrum of irisflorentin. Figure S6: Mass spectrum of afzelechin/epiafzelechin. Figure S4: Mass spectrum of procyanidin B1/procyanidin B3. Figure S3: Mass spectrum of gallocatechin/epigallocatechin. Figure S2: Mass spectrum of catechin or epicatechin. catechu ethyl acetate fraction and red line for A. Figure S1: Total ion chromatogram (TIC) blue line for A. You can save your work and email MNova files.Supplementary Materials: Table S1: Indigenous uses and chemical constituents' description of A.Each spectrum should be annotated according to the guidelines I have provided/shown you.Each time you perform an experiment, it is your responsibility to obtain an H’NMR spectra in order to assess your reaction purity and identify your compound.Check out the following MestReNova Tutorials.Filezilla is free software, search to download a free version.Open MNova and add the three license files you accessed previously.
Do not download the free trail, click on Downloads and download and install the latest MestReNova version for Mac or PC.Click here for a beginner’s guide.ĭownloading and installing MNova and FileZilla on your personal computer:
We use the software Mestrenova (MNova) to assign peaks and analyze our spectra.
Mentors should show students how to prepare a sample and also take the NMRs for the students. To analyze spectra on your personal computer scroll down for instructions. Training is required and given to each student on how to prepare an NMR sample, reserve NMR time, use the instrument, and finally analyze the spectra. The Chemistry Department has a NMR facility with several spectrometers located on the fifth floor of NHB.